عنوان البحث(Papers / Research Title)
Occurrence and detection of extended-spectrum B-lactamases in Klebsiella isolates in Hilla, Iraq
الناشر \ المحرر \ الكاتب (Author / Editor / Publisher)
علاء هاني حسن الجراخ
Citation Information
علاء,هاني,حسن,الجراخ ,Occurrence and detection of extended-spectrum B-lactamases in Klebsiella isolates in Hilla, Iraq , Time 4/28/2011 11:23:09 PM : كلية الطب
وصف الابستركت (Abstract)
دراسة انزيمات البيتالاكتاميز واسعة الطيف في عزلات الكليبسيلا في مدينة الحلة
الوصف الكامل (Full Abstract)
Occurrence and detection of extended-spectrum B- lactamases in Klebsiella isolates in Hilla, Iraq
Alaa H. Al-Charrakh, Samira Y. Yousif and Hussein S. Al-Janabi
Abstract
Extended-spectrum beta-lactamases (ESBLs) have been found in many pathogenic gram-negative bacteria, butthey are most common in nosocomial isolates of Klebsiella pneumoniae, giving a proportion of 75% of ESBL-producing strains.
A total of 88 Klebsiella pneumoniae isolates were recovered from different environmental and clinical samples in Hilla/Iraq during the period from January to July 2003. Primary screening of B-Lactam resistant isolates showed that 73.8% (65 strains) were resistant to -Lactam antibiotics. 58.4% of these strains were B- lactamase-producers. All the B- lactamase-producing Klebsiella strains showed multiple-drug resistance to least 8 antibiotics. Klebsiella strains were also tested for their ability to produce extended-spectrum beta-lactamases (ESBLs) using three methods.
Determination of minimum inhibitoryconcentration (MIC) with and without clavulanate was the most accurate method for detection of ESBLproducingisolates, by which 8 (21%) ESBL-producing isolates were detected. Plasmid profile of Klebsiella strains (including ESBL-producers) was detected.
The genes encoding for the production of ESBLs and resistance to penicillin, ampicillin, amoxicillin, tetracycline, refampin, and erythromycin were located on conjugative plasmids whereas genes encoding for resistance to cephalothin, cefazolin, cephalexin and gentamycin were located on the chromosome.To our knowledge, the present study is the first to investigate the occurrence of ESBLs and detection of genetic factors controlling ESBL production in Klebsiella isolates in the area of the study.
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