Genetic Method for Detection Four Virulence Factors Associated with Enterococcus Faecalis
Abstract
In this study, four virulence genes were investigated in seven clinical isolates of E. faecalis collected from 104 samples by using PCR techniques. Enhanced expression of pheromones (eep) was seen to be present in only 2 isolates which were isolated from urine samples, whereas Enterococcus faecalis endocarditis (efaA) was present in five isolates (3 isolated from urine and 2 from wound).
Besides, internalin AB (inlAB) was also detected. It was found that inlAB is present in 4 isolates (3 urine and one from wound). The enterolysin A (enlA)is absent from all isolates.
The presence of such virulence genes may promote the ability of this bacteria to cause a disease in human.