The objective of this research was to investigate the effect of tocopherol extraction of Lepidium sativum seeds on the fertility of adult male rabbit. The In Vivo study included the estimation of the Medium Effect Dose (MED50) for tocopherol extraction by using Dose-Response Curve. The mean of MED50 was 33.6 mg /kg body weight (b.w.) of tocopherol . The results showed a significant increase (P<0.05) in testicular sperm concentration, epididymus sperm concentration and in the sperm count per gm of the testis, sperm motility percent, grade activity, sperm viability percent .While it was found a decrease in abnormal sperm morphology percent of epididymis caudal at MED50 of tocopherol. Our results reveal that due to tocopherol administration; male rabbits fertility increases, thus being useful to any animal, mammals as well as human being. Lepidium sativum (family Brassicaceae) locally known as ‘garden cress’ has been used widely in different parts of the world for its wide therapeutic application, a number of recent studies pointed out the traditional uses of Lepidium sativum seeds extract in controlling many clinical problems [1], and [2] revealed that Lepidium sativum seeds with high nutritional value can be exploited as a functional food ingredient, and Garden cress meal is a by-product remaining after the extraction of the oil from seeds. It is usually cultivated for its leaves, which are used in salad, sandwiches etc. [3].The leaves and seedpods have a peppery taste, and have been reported to have enormous biological activities [4]. It is documented to possess, tocopherol , phenolic compounds , nitrogen compounds , terpenoids , and some other endogenous metabolites, which are rich in antioxidant activity [5]. Rashad seeds contain two sets of fat-soluble compounds, and represent the first group of Tocopherols, which consists of (21ppm) alpha - tocopherol and (1422ppm) gamma - tocopherol and (35ppm) Sigma – tocopherol, The second group are Tocotrienols [6]. Many natural dietary agents, including vegetables, fruits , herbs, and spices have been used in traditional medicines, as non-conventional treatments, for thousands of years, but without sufficient scientific proofs . If effective, natural agents might lead to the development of natural and novel drugs with low or no side effects [7]. Although vitamin E has been known as an essential nutrient for reproduction since 1922, we far from understand the mechanisms of its physiological functions [8]. Vitamin E cannot be synthesized by humans and must be obtained from the diet with an abundant source found in vegetable oil, nuts, and egg yolks [9]. To date, there are approximately 100 publications on this topic, which highlight the beneficial effects of this antioxidant on viability, membrane integrity and motility of spermatozoa of different species.