ABSTRACT Propolis is a resinous substance collected by worker bees (Apismellifera) from the bark of trees and leaves of plants. The aim of this study is to elucidate the chemical nature of the bioactive compounds isolated from Iraqi propolis using different methods of analysis and Identification of propolis components by TLC. Propolis samples were collected during spring and summer seasons (2014). Phytochemical screening of propolis revealed presence of flavonoids, tannin, alkaloids, mucilage, saponin, fat, terpenoids, volatile oils phenolic and coumarins compounds as a major constituents and absence of glycosides with steroids. The major constituents of propolis revealed that proteins were predominant components followed by carbohydrates, fat, saponin, moisture and ash.The types of amino acids in proteins of Al-Museiabpropolis were proline, hydroxyl proline, tyrosine, tryptophan, arginine, cysteine, cystine, methionine, whereas casein was not present in Al-Museiabpropolis. Chemical screening of Al-Museiabpropolis revealed presence of six fatty acids represented by mgristic acid, palmtic acid, arachidic acid, olic acid, linoleic acid, and linolinic acid. The chemical analysis of mineral elements in Al-Museiabpropolis was also determined; K was predominant in Al-Museiabpropolis followed by Na, Fe, Mg and Ca, while Zn Cu, Co and Ni were presented in low concentration. Also we were isolated some compounds by TLC pates. The result of alcoholic and aquatic extracts of propolis analysis indicated that the contained 4 compounds for each extract. Thus it was concluded that Al-Museiabpropolis have active components as a major constituents and Al-Museiabpropolis were rich with proteins, carbohydrates, fat, and saponin. It has fatty acid and mineral elements. Keywords: Propolis, Phytochemical analysis, Iraq.