The objectives of this study were analysis of the secondary metabolite products from Aspergillus
fumigatus and evaluation antibacterial activity. Fifteen bioactive compounds were identified in the
methanolic extract of Aspergillus fumigatus. The identification of bioactive chemical compounds is based
on the peak area, retention time molecular weight and molecular formula. GC-MS analysis ofAspergillus
fumigatus revealed the existence of the 9-Hexadecenoic acid ,2,4-Dimethyl-5-methylthiopent-4-en-2-ol ,
E-11-Hexadecenoic acid , ethyl ester , D-Glucose , 6-O-?-D-galactopyranosyl- , ?-D-Glucopyranoside ,
O-?-D-glucopyranosyl-(1.fwdarw.3)-?- , 6-Acetyl –?-d-mannose , 4H-Pyran-4-one,2,3-dihydro-3,5-
dihydroxy-6-methyl- , 5-Hydroxymethylfurfural , ?-D-Glucopyranoside, methyl , Tetraacetyl-d-xylonic nitrile
, n-Hexadecanoic acid , 9-Octadecenoic acid , (2-phenyl-1,3-dioxolan-4-yl)methyl ester , Octadecanoic
acid ,9,10-Secocholesta-5,7,10(19)-triene-3,24,25-triol,(3?,5Z,7E)- and Pyrimidin-2-ol,4-(3,4-
dimethoxyphenyl)-6-phenyl. Gramineae poaceae was very highly antifungal activity (8.32±0.09) mm while
Streptococcus faecalis has a maximum zone formation (6.92±0.24) mm against Aspergillus fumigatus.