عنوان البحث(Papers / Research Title)
Analysis of mtDNA hypervariable region II for increasing the discrimination power from Middle and South of Iraq
الناشر \ المحرر \ الكاتب (Author / Editor / Publisher)
عماد هادي حميد الطائي
Citation Information
عماد,هادي,حميد,الطائي ,Analysis of mtDNA hypervariable region II for increasing the discrimination power from Middle and South of Iraq , Time 30/12/2016 14:05:01 : كلية التمريض
وصف الابستركت (Abstract)
Samples of 320 random healthy unrelated Iraqi male persons from the Arab ethic group of Iraqi population were collected for Y chromosomal STRs typing. FTA® Technology was utilized to extract DNA from blood collected on FTA™ paper. We analyzed 11 Y chromosomal STR loci for evaluating allele frequencies and gene diversity for each Y-STR locus of the Y-Plex™ PCR amplification kit. The 11 loci include DYS389I, DYS389II, DYS19, DYS391, DYS438, DYS390, DYS439, DYS392, DYS393, DYS385a and DYS385b. A total of 299 unique haplotypes was identified among the 320 individuals studied. The DYS385b had the highest diversity (GD = 0.8392), while loci DYS392 had the lowest (D = 0.2695). The study focuses to establish the basic forensic genetic informations, knowledge, data and statistics which might be so ultimately helpful practically in forensic science and criminology and to let evaluate and present the DNA weight evidences in Iraq medico-legal institute and courts of law.
الوصف الكامل (Full Abstract)
Mitochondrial DNA is a useful genetic marker for answering evolutionary questions due to its high copy number, maternal mode of inheritance, and its high rate of evolution. The aims of this research were to study the mitochondria noncoding region by using the sanger sequencing technique and establish the degree of variation characteristic of a fragment FTA® Technology (FTA™ paper DNA extraction) utilized to extract DNA. A portion of a non-coding region encompassing positions 37 to 340 for HVII was amplified in accordance with the Anderson reference sequence. PCR products were purified by EZ-10 spin column then sequenced and detected by using the ABI 3730xL DNA analyzer. New polymorphic positions G92C, C113G, C150G, T156A, C194G, C198G, G207C, G225C and G228C are described and may in future be suitable sources for identification purpose. The data obtained can be used to identify variable nucleotide positions characterized by frequent occurrence most promising for identification variants.
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