عنوان البحث(Papers / Research Title)
Detection of novel polymorphisms in the mitochondrial DNA D-Loop hypervariable region HVI from 400 healthy unrelated individuals from central and North-central Iraq
الناشر \ المحرر \ الكاتب (Author / Editor / Publisher)
عماد هادي حميد الطائي
Citation Information
عماد,هادي,حميد,الطائي ,Detection of novel polymorphisms in the mitochondrial DNA D-Loop hypervariable region HVI from 400 healthy unrelated individuals from central and North-central Iraq , Time 30/12/2016 14:15:00 : كلية التمريض
وصف الابستركت (Abstract)
The mitochondrial DNA (mtDNA) is a small circular genome located within the mitochondria in the cytoplasm of the cell and a smaller 1.2 kilobase pair fragment, called the control region (D-loop).
الوصف الكامل (Full Abstract)
The mitochondrial DNA (mtDNA) is a small circular genome located within the mitochondria in the
cytoplasm of the cell and a smaller 1.2 kilobase pair fragment, called the control region (D-loop). The
aims of this research were to study this region by using the Sanger sequencing technique and establish
the degree of variation characteristic of a fragment. FTA® Technology (FTA™ paper DNA extraction)
was utilized to extract DNA. PCR products were purified by EZ-10 spin column then sequenced and
detected by using the ABI 3730xL DNA Analyzer. Novel polymorphisms discovered at positions 16037,
16075, 16104 and 16201 in future may be suitable sources for identification purpose.
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