The objectives of this study were to analyze the secondary metabolites of Penicillium expansum and
evaluate antibacterial activity. Twenty eight bioactive compounds were identified in the methanolic
extract of P. expansum. The identification of bioactive chemical compounds is based on the peak area,
retention time molecular weight and molecular formula. Gas chromatography mass spectrometry
(GC/MS) analysis of P. expansum revealed the existence of the Levoglucosenone, Edulan ll, 4-
[Dichloromethyl]-2-[[2-[1-methyl-2-pyrrolidinyl]ethyl]amino-6-trichloro, 1,2-Cyclopentanedione,
Ethanethiol, 2-(5-(4-methyl-2-pyridyloxy)pentyl)amino-hydrogen su, Imidazole,2-amino-5-[(2-
carboxy)vinyl], D-Glucose,6-O-?-D-galactopyranosyl, Eicosanoic acid , phenylmethyl ester, Dodecanoic
acid, 3- hydroxyl, DL-Leucine,N-glycyl, Cyclohexene,1,5,5-trimethyl-6-acetylmethyl, 1,2-Nonadecanediol,
Bicyclo[2.2.1]heptane-2-carboxylic acid isobutyl-amide, 6-Acetyl-?-d-mannose, ?-DGlucopyranoside,
O-?-D-glucopyranosyl-(1.fwdarw.3)-?-D-fruc, propanedioic acid, amino-diethyl ester,
4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl, valeric acid, dodecyl ester, deoxyspergualin, IGala-
I-ido-octonic lactone, 5-Hydroxymethylfurfural, paromomycin, 16-Nitrobicyclo[10.4.0]hexadecane-
1-ol-13-one, cis-Vaccenic acid, 2-Bromotetradecanoic acid, phthalic acid, butyl undecyl ester,
picrotoxin, D-Fructose and diethyl mercaptal. The FTIR analysis of P. expansum proved the presence of
aliphatic fluoro compounds, tetiary amine, C-N stretch and methylene-CH. asym which shows major
peaks at 1028.06, 1151.50 and 2852.72, respectively. Methanolic extract of bioactive compounds of P.
expansum were assayed for in vitro antibacterial activity against Proteus mirabilis, Pseudomonas
aerogenosa, Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia by using the diffusion
method in agar. The zones of inhibition were compared with different standard antibiotics. S. aureus
has maximum zone formation (7.01±0.141) mm.