عنوان البحث(Papers / Research Title)
Discovery of Three Newly Described Single Nucleotide Polymorphisms in Mitochondrial DNA Hypervariable Region I (HVI) and Estimation of Variants and Haplotypes Encompassing Nucleotide Positions 16024- 16365
الناشر \ المحرر \ الكاتب (Author / Editor / Publisher)
عماد هادي حميد الطائي
Citation Information
عماد,هادي,حميد,الطائي ,Discovery of Three Newly Described Single Nucleotide Polymorphisms in Mitochondrial DNA Hypervariable Region I (HVI) and Estimation of Variants and Haplotypes Encompassing Nucleotide Positions 16024- 16365 , Time 30/12/2016 14:28:13 : كلية التمريض
وصف الابستركت (Abstract)
The aims of this research are to study the mitochondria noncoding region by using the Sanger sequencing technique and establish the degree of variation characteristic of a fragment.
الوصف الكامل (Full Abstract)
The aims of this research are to study the mitochondria noncoding region by using the Sanger sequencing
technique and establish the degree of variation characteristic of a fragment. FTA® Technology (FTA™ paper DNA
extraction) utilized to extract DNA. A portion of a noncoding region encompassing positions 16024-16365 amplified
in accordance with the Anderson reference sequence. PCR products purified by EZ-10 spin column then sequenced
and detected by using the ABI 3730xL DNA Analyzer. The most frequent variant differed in the single position
A16227G, according to the CRS sequence. A new polymorphic position 16046, 16105 and 16141 are described may
in future be suitable sources for identification purpose. The data obtained can be used to identify variable nucleotide
positions characterized by frequent occurrence most promising for identification variants.
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