X-Chromosome short tandem repeat (STR) typing can complement existing DNA profiling protocols and
can also offer useful information in cases of complex kinship analysis. This is the first population study
of 8 X-linked STRs in Iraq. The purpose of this work was to provide a basic data of allele and haplotype
frequency for x-linked markers and methods that are commonly used to analyze microsatellites, and the
subsequent possibilities of using these specifities especially in forensic genetics. FTA® Technology
(FTA™ paper DNA extraction) was utilized to extract DNA. Amplification was performed using the
Mentype® Argus X-UL PCR amplification kit. Products were detected using ABI PRISMR 3100 Genetic
Analyzer (Applied Biosystems). Forensic efficiency parameters showed that DXS7132, DXS7423,
DXS8378, HPRTB, DXS10074, DXS10101, DXS10134 and DXS10135 are suitable for forensic application
in Iraq. All the analyzed markers were in Hardy-Weinberg equilibrium (HWE); therefore Hardy-Weinberg
laws could be applied for match probability calculation