عنوان البحث(Papers / Research Title)
Sequence analysis of mitochondrial DNA hypervariable region III of 400 Iraqi volunteers
الناشر \ المحرر \ الكاتب (Author / Editor / Publisher)
عماد هادي حميد الطائي
Citation Information
عماد,هادي,حميد,الطائي ,Sequence analysis of mitochondrial DNA hypervariable region III of 400 Iraqi volunteers , Time 30/12/2016 18:53:15 : كلية التمريض
وصف الابستركت (Abstract)
The aims of this research were to study mitochondrial DNA hypervariable region III and establish the degree of variation characteristic of a fragment
الوصف الكامل (Full Abstract)
The aims of this research were to study mitochondrial DNA hypervariable region III and establish the degree of variation characteristic of a fragment. The mitochondrial DNA (mtDNA) is a small circular genome located within the mitochondria in the cytoplasm of the cell and a smaller 1.2 kb pair fragment, called the control region (D-loop). DNA was extracted from blood following a standard phenolchloroform method. Polymerase chain reaction (PCR) products were purified by EZ-10 spin column then sequenced and detected by using the ABI 3130xL DNA analyzer. New polymorphic positions detected as T460A, C426G, T471A, T482A, A493T and C518G may in future be suitable sources for identification purpose.
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