effectiveness of aqueous and alcoholic extract of” annona squamosa“ plant against some types of gram positive and negative bacteria.
iman fadhil abdul-husin1, nadhim mshtaq hashim2, nada khazal kadhim hindi3,
fatima malik abood4, and ammar khazaal kadhim5.
1msc in microbiology, college of biotechnology, al-qasim green university, babylon province, iraq.
2phd in microbiology, college of biotechnology, al-qasim green university, babylon province, iraq.
3phd in microbiology, basic and medical science department, college of nursing, babylon university, babylon province, iraq.
4phd in microbiology, college of dentistry, babylon university, babylon province, iraq.
5msc in microbiology, babylon province, iraq.
abstract
the attention to plants and medicinal herbs has increased. it use as key sources for the production of medical drugs or as a source of effective materials that go into the composition of the drug.in order to determine the medical significance of annonasquamosa plant and its anti-microbial activity against some types of gram positive and negative bacteria by means measure the diameter of the inhibition zone of it by aqueous and alcoholic extract of each parts of fruit, where make water extract and methanol extract from pulp, shell and seed of the” apple honey”.these bacteria were isolated from clinical samples of people whom fallen asleep in al-hilla teaching hospital, babylon during a period from october2015 to january 2016. in the present study the diagnosis of bacterial strains where after its culturing on suitable mediaand then the diagnosis was performed by using vitek 2 system to identify species level of isolates strains. these strains were included aeromonashydropinghila, klebsiella pneumoniae, escherichia coli, streptococcus feacalis. staphylococcusaeurus, pseudomonas aeruginosa, and salmonella paratyphi .in order to detection antibacterial activity of aqueous and alcoholic extract of annona squamosa.the methanolic extracts of annonasquamosahave higher antimicrobial activity than aqueous extract seed and fruit of present plant, while the shell of the extract have no activity on studied bacteria . also higher diameters of inhibition zone was to alcoholic extract against escherichia coliwere (26) mm at the concentration was (50) mg \ ml, while less diameters of inhibition zone wasin klebsiellapneumoniae10 mm in the concentration (50) mg \ ml in of aqueous extract of the seed. from the result we concluded the a. squamosa extracts has beneficial effect onpathogenic bacteria.the methanol and water extracts of seed have better activity than other extracts that can be used to prevent enteric diseases.
keywords:annonasquamosa, antimicrobial activity, agar discdiffusion method, ethanol, aqueous extract.