Effect of N-Acetyl-l-Cycteine on the Growth and the Antibiotic Resistance of both Pseudomonas aeurginosa and Klebsiella pneumoniae
 
Lamees Abdul Razzak Abdul LateefDept. of Microbiology College of Medicine/Babylon University 
 

Abstract
In this study, Pseudomonas aeurginosa and Klebsiella pneumoniae were obtained from patient with burn wound infection in Hilla surgical teaching hospital. The effect of NAC on the growth of Pseudomonas aeurginosa and Klebsiella pneumoniae bacteria was investigated. It was found that NAC at concentrations ranging from (0.4-1)mg/ml cause an inhibition to Pseudomonas aeurginosa and Klebsiella pneumoniae growth.
Pseudomonas aeurginosa was resistant to all the antibiotics, while Klebsiella pneumoniae was sensitive to kanamycin and streptomycin but resistant to other antibiotics before treatment with NAC.
On the other hand, the combination effect of NAC(at concentration 0.01mg/ml) and antibiotics on the bacterial growth was also studied. The results showed that Klebsiella pneumoniae was sensitive to kanamycin and streptomycin but resistant to other antibiotics, the zone of inhibition is reduced after addition of NAC compared with the zone without NAC. While Pseudomonas aeurginosa entirely resistant to the antibiotics after and before the addition of NAC.
 
Keywords:
 
N-Acetyle-l-cycteine, Psudomonas auroginosa, Klebsiellia pnuemonia.