Biotechnology and Biochemical Analysis Technique to isolate Vanillin derivatives for Plum fruit Tree (I)


Abstract :
It’s well known that alternative medicine depends mainly on herbs and medical plants in the hall world which in turn have a lot of benefits in the field of medical treatments through what they have different types of cyclic organic molecules.
This application studied some biochemical and technological techniques to identify ,isolate and purified some of biochemical molecules from fruits of Plum tree .
The study involved drying the sample with freeze dryer for 24 hrs . protic solvent (PSI) , aprotic solvent (ASI) ,and aprotic–protic solvent interaction( PASI) were used to extracted the molecules then directly transformed to the flash column chromatography (FCC)and monitored by Thin layer chromatography (TLC) to isolate m-methoy-p-hydroxy benzoic acid (vanilic acid) and m-methoxy-p-hydroxy ethyl Benzoate ) in pure state. These molecules elucidate by spectroscopic tools like ( UV-Vis , ATR-IR ,1H-NMR and LC –Mass) .


Key words : plum tree , Vanilinic acid ,flash chromatography , purification .

Corresponding author e.mail : ajmbioorg@gmail.com