ABSTRACT

The objectives of this study were analysis of the secondary metabolite products and evaluation antibacterial
and antifungal activity. Bioactives are chemical compounds often referred to as secondary metabolites.
Twenty nine bioactive compounds were identified in the methanolic extract of Citrus aurantifolia. The
identification of bioactive chemical compounds is based on the peak area, retention time molecular weight
and molecular formula. GC-MS analysis of Citrus aurantifolia revealed the existence of the Thieno[2,3-c]
furan-3-carbonitrile, 2-amino-4,6-dihydro-4,4,6,6, Furfural, 2-Vinyl-9-[3-deoxy-?-d-ribofuranosyl]
hypoxanthine, 2-Myristynoyl pantetheine, 2,5-Furandione, dihydro-3-methylene-, Cyclohexene,1-methyl-
4-(1-methylethenyl)-,(S)-, O-Acetyl-4-hydroxyproline, 1,5,5-Trimethyl-6-methylene-cyclohexene, Acetic
acid, 2-(1-buten-3-yl)-2-nitro-,ethyl ester, Methyl 3-hydroxytetradecanoate, L-?-Terpineol , 4-Methyl
itaconate Glycyl-D-asparagine, 2(3H)-Benzofuranone , hexahydro-7a-methyl- , 7-Oxa-2-oxa-7-
thiatricyclo[4.4.0.0(3,8)]decan-4-ol, Cholestan-3-ol , 2-methylene-,(3?,5?)- , Formic acid , 3,7,11,-trimethyl-
1,6,10-dodecatrien-3-yl ester , 7-epi-cis-sesquisabinene hydrate , 2,5-Cyclohexadien-1-one , 3,5-dihydroxy-
4,4-dimethyl-2-(1-oxo , Pyrrolidin-2-one-3?-(propanoic acid, methyl ester), 5-methylen, D-Fructose, diethyl
mercaptal , pentaacetate, n-Hexadecanoic acid, 2H-1-Benzopyran-2-one, 5,7-dimethoxy- , Dihydroxanthin,
Oleic acid, Octadecanoic acid , Phorbol , 9-Octadecenamide, (Z)- and 9-Octadecenamide.Clinical pathogens
were selected for antibacterial activity namely, Staphylococcus aureus, Escherichia coli, Proteus mirabilis,
Klebsiella pneumonia, and Pseudomonas eurogenosa. Citrus aurantifolia has maximum zone against
Escherichia coli 5.66±0.21.

INTRODUCTION

Fruit a globose to ovoid berry, 3-6 cm in diameter,sometimes with apical papillae, greenish-yellow; peelvery thin, very densely glandular; segments with yellowgreen pulp-vesicles, very acid, juicy and fragrant. Seeds small, plump, ovoid, pale, and smooth with white embryos (polyembryonic). The fruit is used in nearly every home in the tropics, mainly to flavour food, but also to prepare drinks and for a variety of medicinal applications. The antifungal activity of the plant have been attributed to the presence of monoterpenes and the plant is currently used as a fungicide for citrus fruit crop, and it has also been suggested that the plant may be a potential candidate used for the protection of food and feeds from toxigenic fungal growth as well as their aflatoxin contamination 1-9. It has been traditionally used in the management of several diseases and has the prospects of being developed into useful drugs. Citrus fruits are highly recommended for persons suffering from kidney stones, gout and arthritis 10-18. C. aurantifolia juice contains potassium citrate which prevents the formation of kidney stones and eases their dissolution. Due to the high content of vitamin C, citrus fruits are used in the treatment of scurvy 19-23. The aims of our study were analysis of the metabolite products and determination of antimicrobial activity.