Abstract: This application studied some biochemical and technological techniques to identify, isolate and purify some of the biological molecules from fruits of a Plum tree . The study involved drying the sample with a freeze dryer for 24 hrs. Then samples were performed with Soxhlet apparatus by protic solvent (PSI), aprotic solvent (ASI), and aprotic–protic solvent interaction( PASI) were used to extract the molecules. Then recovered the excess solvent evaporation and condensation. The sample directly transformed to the flash column chromatography technique (FCC)and monitored by Thin layer chromatography (TLC) to isolate m-methoy-p-hydroxybenzoic acid (vanillic acid) or (VA) and m-methoxy-p-hydroxy
ethyl Benzoate (Ethyl Vanillate) or EV in about 95% pure state. These molecules AV and EV elucidated by spectroscopic tools (UV-Vis, ATR-IR, 1H-NMR, 13C-NMR, and Mass spectrometry).