Objective Hospital environment can represent an important source of pathogens which high risk for
patients. Acquired antimicrobial resistances is the major problem .Aims of this study to detection the
common contaminant bacteria among the hospital environment and determine the antimicrobials activity
of agents (Apple vinegar, Garlic vinegar, Dates vinegar, Raisin vinegar) against pathogenic bacteria
isolated from hospital environment. Method In the study 172 samples were taken by swabs from three
hospitals in Hilla city, from October 2016 to march 2017.Antimicrobial activity test of (apple, palm, garlic
and black raisins) vinegar was determined by MIC and Agar-well diffusion assay, alsoantibiofilm
Formation and ant adherence activity of vinegar against microbes were determined. Result In this study,
172 samples were taken from three Hospitals in Hilla city the result showed that 73 (42.44%) samples
were positive cultured and 99 (57.55%) negative cultured from total samples. Staphylococcus aureus37
(33%) was highly isolated from other microbes followed by Bacillus anthrax. Conclusion Staphylococcus
aureus is more common in Hospitals in Babylon Provence, vinegar is the most effective substance that
killing bacteria that contaminate hospital environment.