Abstract
Silver nanoparticles is widely applied in recent years. These particles have minimal effects on FSH yet, they raise levels of
LH hormone. Human chorionic gonadotropin hCG has a similar structure and function as LH. This hormone is used in
ovulation induction in human and animals but, it may involve in the formation of cystic follicle. The aim of this study is to
examine the effect of silver NPs on some physiological changes in ovaries of female mice treated previously with hCG.
Mature female mice (32) in number were divided into the following groups (n=8) for each: G control which was excluded
from treatment, G (hCG) treated by intraperitoneal injection with hCG solution(10 I.U) three times a week for two weeks,
then they were sacrificed and ovaries were isolated and preserved for histological study. The rest(16), were divided into
two groups according to the concentration of silver NPs solution(0.5 and 1ppm) which was administrated by (i.p) injection
twice a week for 45 days. At the end of this period, isolated ovaries were sectioned and stained with routine stains
haematoxylin and eosin. A significant increase (P?0.05) in diameters of ovaries for both groups treated with hCG then with
(0.5 and 1ppm) of silver NPs. Number of pre-antral follicles were significantly high(P?0.05) in the group treated with
hCG+ 0.5 ppm of silver NPs. Number of antral was significantly high in the group treated with hCG. Whereas, number of
corpora lutea in the group treated with hGC+ 1ppm silver NPs was significantly higher (P?0.05). All the histopathological
changes in the treatments were due to silver NPs.
Keywords: hCG, Cystic Follicle, Silver NPs, Corpus Luteum Cyst