Antimicrobial resistance of bacteria is one of the serious problems that appears in wound healing and may result in
difficulties such as reduction in healing time or inflammations. Piezoelectric materials might be utilized in
producing bioactive electrically charged surface. This paper focuses on producing two groups of bio-composite from
polyvinylidene fluoride with addition of Nano-magnesium oxide (3, 5 and 7 %) wt. by two methods: electro-
spinning and spin coating under special condition, with the aim of producing bio film as sensor for wound healing.
The Antibacterial test was illustrated by Agar well dispersion strategy for three groups of the prepared film,
reflecting the ability of n-MgO/PVDF biofilm to prevent the bacterial growth. The best sample that showed the
greatest inhabitation zone was 7 wt.% n-MgO for both types of bacteria, in contrast with spin coated samples, which
did not give any results for both type of bacteria. The AFM revealed topography of nanofiber (PVDF /n-MgO), which
appeared like hills as compared to the same sample prepared by spin coating. DSC test showed that the increment
of n-MgO to PVDF brought about a diminishing in the enthalpy for fusion (?Hm), suggesting a reduction in the
crystallinity in PVDF. From contact angle test, it can be seen that the pure PVDF nanofiber showed water contact
angle (WCA) of ?108?, which showed the hydrophobic nature. The WCA is enhanced by presenting MgO and
increasing MgO content. The WCA showed enhancement in increasing hydrophilic action where nanofiber (PVDF
/n-MgO) showed WCA of 120? diminishes to 41?and the sliding angle diminishes displaying super- hydrophilicity.
Consequently, the antibacterial action of the arranged nanofiber tests was improved, when contrasted with the
counterpart spin coated membrane. Morphological analysis of first group demonstrated the presentation of random
Nano fiber with some agglomeration of nano-MgO, where these agglomerates diffuse through agar plate and cause
inhabitation of bacterial growth, while the second group shows smoother and flattened surface topography where
the morphology appeared as a ringed-spherulitic matrix. FTIR studies reveal the presence of piezoelectric ?–phase
at 840cm-1 wave. As a result, the piezoelectric properties are found in the electrospun PVDF/n-MgO nanofiber which
is necessary for advancement of biosensors used in sensing wound healing