In the current study collect 18 samples from smoking patient with gingivitis and 7 samples from healthy people as control (age 20-25 years)
to study parasites which found in mouth spatially in gingivitis and effected on IL-2 and IL-4, IgA and IgG we found parasite Entamoeba
gingivalis and Trichomonas tenax. 10 samples have Trichomonas tenax and 8 samples have Entamoeba gingivalis. In other hand we found
significant differences at 0. 05 in IL-2 (Mean =20.62727 infected with Trichomonas tenax, Mean =19.31818 infected with Entamoeba
gingivalis, control =11.4), IL-4 (Mean =509.3636 infected with Trichomonas tenax, Mean=235.2727 infected with Entamoeba gingivalis,
control=7), IgA (Mean =264.50 infected with Trichomonas tenax, Mean=243.50 infected with Entamoeba gingivalis, control = 7) and IgG
(Mean =975.00 infected with Trichomonas tenax, Mean = 1024.00 infected with Entamoeba gingivalis, control = 7) which mean this
parasitical infection increase periodontitis additional to smoking. Gingivitis may happened by parasites not by bacteria we found IgG level
had been decreased in while IgA, IL-2 and IL-4 levels were increased in serum during infection with parasite.
Key words: Entamoeba gingivalis, Trichomonas tenax, IL-4 , IL-2, IgA and IgG.