Amniotic fluid is defined as a transparent liquid that is present in the uterus during
pregnancy. In this study obtain 100 sample of vaginal swaps(50 from normal birth and 50 from
caesarean), 100 samples of amniotic fluid (50 samples from normal birth and 50 samples from
caesarean) from pregnant women revision born lounge in Hilla teaching hospital, and 50
samples of amniotic sac from the caesarean women to made histological section. The result
showed the number of positive swaps of bacteria was 81 (81%) , while positive samples from
amniotic fluid was 74 (74%) from the total 100 sample and present relationships between
vaginal infection and the present of bacteria in the amniotic fluid .The type of aerobic
bacteria isolated was Escherichia coli ,Pseudomonas aeruginosa, Bacillus subtilis, Klebsiella
pneumonia, Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus mutans,
Proteus mirabilis and anaerobic bacteria was Peptostreptococcus sp. Neisseria gonorrhoeae,
Lactobacillus sp. and Actinomyces sp. were isolated from amniotic fluids and vaginal swabs.
then injection the same bacteria isolated in mice and made histological section from mice
amniotic sac. Cross section of amniotic tissues in both of women and mice showed infiltration of
lymphocyte, hemorrhage and elongation of epithelial cell as a result of bacterial infection