Escherichia coli (E. coli) is the most common gram-negative organism causing meningitis during the
neonatal period. Neonatal meningitis due to E coli K1 is a serious illness with unchanged morbidity and
mortality rates for the last few decades. The mechanism involved in the pathogenesis of E. coli meningitis
remains unclear. Experimental studies with animal models have given new insights into its pathophysiology
during the acute phase of the disease . E. coli strain originally isolated from the Cerebrospinal fluid of a child
with 20 days old was complaining acute meningitis. In the present work, we describe in detail a simple,
reproducible and efficient method to induce E.coli meningitis in mice by using the three route (intrapretonium ,
intravenous, oral) of infection . E.coli were recovered of same characters of the original isolate used for
preparing the infective dose. The histopathological changes found in the experimentally infected mice we can
conclude induce meningeal thickening , neutrophiles infiltration , hemorrhages and leukocyte infiltration in
brain