عنوان البحث(Papers / Research Title)
A study of the immunomodulatory effects of substituted ceramide in a rabbit model of induced rheumatoid arthritis
الناشر \ المحرر \ الكاتب (Author / Editor / Publisher)
رعد نعمة جاسم الجبوري
Citation Information
رعد,نعمة,جاسم,الجبوري ,A study of the immunomodulatory effects of substituted ceramide in a rabbit model of induced rheumatoid arthritis , Time 6/15/2011 9:21:40 AM : كلية الصيدلة
وصف الابستركت (Abstract)
The highly progressive arthritic process in rheumatoid arthritis renders the disease a chronic
الوصف الكامل (Full Abstract)
A study of the immunomodulatory effects of substituted ceramide in a rabbit model of induced rheumatoid arthritis
Abstract
The highly progressive arthritic process in rheumatoid arthritis renders the disease a chronic and crippling illness that requires chronic anti-inflammatory and immunosuppressive drugs at the same time patient activity is highly deteriorated. This means a high economic and health burden from the disease itself and the drugs.
On the other hand, immune modulating natural products can be implemented as a safe and accessible treatment for autoimmune diseases after evaluating their potency as an alternative solution to overcome that health problem.
Ceramide is a sphingolipid that is widely distributed among dietary plant parts including wheat flour and rice bran in addition to soybeans seeds and potato peel.
It is also de novo biosynthesized and being a major structural and functional consistent of the cell membrane especially in neuronal and epidermal cells, however, ceramide is considered as an essential second messenger in transducing cell cycle regulation through both direct gene control and indirectly by modifying different cytokines like interleukin 1,2,4,6 and 10 and TGF alpha. Activities of different cells including lymphocytes and macrophages (the major contributor cells of arthritis) will be modified by ceramide.
In a trial to evaluate anti-arthritic activity of ceramide in this study, it was extracted chemically from soybean seeds and identified by HPLC to be evaluated in a rabbit model of knee joint arthritis.
A twenty-four local domestic male rabbits were divided into 4 groups; healthy control, induced untreated control and azathioprine treated control in addition to ceramide treated group.
They were induced with knee joint arthritis by intra-articular injection of 2 doses of bovine albumin plus 40% formalin 7 days apart.
They were assessed for their knee joint local inflammatory signs in addition to every 10 days measurement of ESR, total serum protein, microscopic assessment of peripheral neutrophils and lymphocytes activities as well as immunoassay monitoring of serum interleukin 4 and IL10.
After the 30 days treatment course, the rabbits were autopsied for their histopathological assessment of their knee joints.
Correlation between the cumulative effects of ceramide and the potency of improval of a parameter was estimated.
There was a significant reduction of the knee joint circumference as an indicator of swelling was obtained with ceramide and azathioprine as compared with untreated group; P<0.05.
There was a significant reduction in the percentage of active peripheral neutrophils that shows hypergranulated cytoplasm and hyperlobulated nuclei was obtained with ceramide and azathioprine as compared with untreated group; P<0.05.
There was a significant decline in the fraction of blood lymphocytes count that have a significant increase in cytoplasmic fluorescent active substances was obtained with ceramide and azathioprine as compared with untreated group; P<0.05.
There was non significant changes in ESR vales obtained with ceramide and azathioprine as compared with untreated group at P<0.05.
There was no significant correlation change in total serum proteins in ceramide and azathioprine treated groups as compared with untreated group at P<0.05.
There was a significant reduction in serum IL-4 concentration abone azathioprine treatment, while, ceramide accompanied by non significant changes in IL-4 concentration at P<0.05.
There was a significant raise in serum IL-10 concentration obtained with ceramide treatment, whereas azathioprine shows non significant changes in IL-10 concentration at P<0.05.
There was a significant left histogram shift in grades of arthritis obtained with ceramide and azathioprine treatment as compared with untreated group (P<0.05), ceramide more potent histopathologically than azathioprine.
As a conclusion; there is a promising significant anti-arthritic activity for ceramide that can improve the outcome of rheumatoid arthritis treatment.
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