عنوان البحث(Papers / Research Title)
Study on Cysteine Proteinase Produced by Entamoeba Histolytica
الناشر \ المحرر \ الكاتب (Author / Editor / Publisher)
هيام خالص عنفوص المسعودي
Citation Information
هيام,خالص,عنفوص,المسعودي ,Study on Cysteine Proteinase Produced by Entamoeba Histolytica , Time 7/2/2011 9:10:33 AM : كلية الطب
وصف الابستركت (Abstract)
Study on Cysteine Proteinase Produced by Entamoeba Histolytica
الوصف الكامل (Full Abstract)
Study on Cysteine Proteinase Produced by Entamoeba Histolytica
Mohammad Sabri A. Razzak Souhaila H. Mahmood Hayam Khalis Al-Masoudi
College of Medicine, University of Babylon, Hilla, Babylon, Iraq.
Abstract:
Cysteine proteinases are a large group of enzyme which include lysosomalcathepsins.Cysteine proteinase is considerd to be a key virulence factor which lysis the target cell. Infection with E.histolytic can by controlled by inhibition the virulence factors especially cysteine proteinase . cysteine proteinase produced from trophozoite of E. histolytica have the ability to overcome the defence barrier of colonic epithelia by degrade mucin and invasive to epithelium layer.The action of cysteine proteinase can be regulated and inhibited by endogenous, natural inhibitors as well as compartmentalization and surrounding condition such as pH or chelating agents.
In this work , cysteine proteinase produced by Entamoeba histolytica trophozoite had been detected. The isolated enzyme had been immobilized by using calcium alginate,and compaired between free enzyme (mobile) and immobilized enzyme through differente range of pH and tempreture. We found that immobilized enzyme remains with its enzymatic activity through changing in pH level and temprature in compairion with free enzyme.
Keywords:
Cysteine Proteinase , Entamoeba Histolytica
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