In this work three researchers participate . they are ( ALAA J. MAHRATH*, GHAFIL S. HASSAN and EHAB K. OBAID)
(*)Biochemistry Department, Faculty of Medicine, Babylon University, BABYLON, IRAQ
(a)Physiological Department, Faculty of Medicine, Babylon University, BABYLON, IRAQ
(b)Agriculture College, Al-Qasim Green University, HILLA, IRAQ
ABSTRACT
The present work includes separation and identification of triterpenoids like sterol, betulinic acid
or oleanolic acid extracted from Alhagi roots plant by three stages. The first one involved extraction with
soxhlet and identification process of the crude extract using thin layer chromatography (TLC) technique
that depends on difference in the polarity of solvents of the mobile phase, while the second stage was
isolation of the oleanolic derivative by flash column chromatography (FCC). The final stage includes full
elucidation of isolated component by spectroscopic analysis as Fourier Transformer Infra Red (FT-IR with
KBr disk and ATR mode), 1H-NMR, MS, and Elemental Analysis (C:H:N).