Methanolic extract of bioactive compounds of Callosobruchus maculutus was assayed for in vitro anti-fungal activity against Aspergillus niger, Aspergillus terreus, Aspergillus flavus, Aspergillus fumigatus, Candida albicans, Saccharomyces cerevisiae, Penicillium expansum, and Trichoderma viride. GC-MS analysis of Callosobruchus maculutus revealed the existence of the 4-Cyclooctene-1-carboxaldehyde, Benzenemethanol, 2-(2-aminopropoxy)-3-methyl, 1-(1,4-cyclohexadienyl)-2-methylaminopropane, 2-Undecanone, 6,10-dimethyl-, Tricyclo[10.2.2.2(5,8)octadeca-5,7,12,14,15,17-hexaene, 1-Dimethyl(pentafluorophenyl)silyloxycyclopentane, 3-Methylene-bicyclo[3.2.1]oct-6-en-8-ol, Benzenesulfonamide, N-(bicyclo[2.2.1]hept-5-en-2-ylmethyl), (-)-Norephedrine, 4-Bromo-7-methylenebicyclo[4.2.0]oct-2-ene, Benzene, 1-propenyl-, 1-Hexanol, 2-ethyl-, 1,2-Benzisothiazol-3-amine tbdms, 5-Methyl-6-phenyltetrahydro-1,3-oxazine-2-thione, Propanamide, N-(3-methoxyphenyl)-2,2-dimethyl, 3,6-Octadecadiynoic acid, methyl ester, 1-Chloroundecane, 10-(Tetrahydro-puran-2-yloxy)-tricyclo[4.2.1.1(2,5)]dec-7-en, Pent-1-en-3-one, 1-(2-furyl)-5-dimethylamino, Methyl salicylate, 2-Amino-nicotinic acid methyl ester, 2,5-Dimethylhexane-2,5-dihydroperoxide, 1,4-Oxathiane, 4-oxide, 3-Cyclohexene-1-propanal, Cyclobutane(1,6)spiro(2,3-diazabicyclo[3.1.0]hex-2-ene)-4-, Trans-8-Hydroxy-bicyclo(4,3,0)non-3-ene, Tricyclo[3.3.1.1(3,7)]decane, 1-[(hydrazinocarbonyl)amino]-, (7R)-cis-bicyclo[4.3.0]-3-nonen-7-ol, Ethaneperoxoic acid,1-cyano-1,4-diphenylpentyl ester, 3-Amino-3-(4-isopropoxy-3-methoxy-phenyl)-propionic acid, Aziridinone, 1,3-bis(tricycle[3.3.1.1(3,7)]dec-1-yl), Hydrazinecarboxamide, 2-(2,6-cyclooctadien-1-ylidene, Cis-pinen-3-ol, Salicylaldehyde, thiocarbazone, Adamantane,1-isocyano, 2,7-Methanonaphthalen-3-amine, 1,2,3,4,4a,7,8,8a-octahyd, Pteridine-8-oxide, 6-aldoximino-2-amino-4(3H)-oxo-, 2-Dodecenal, 5H-Cyclohepta-1,4-dioxin,2,3,4a,6,7,9a-hexahydro-,cis-, Hexadecanoic acid, methyl ester, Cyclohexanebutanoic acid, 2-methyl-3-oxo-, methyl ester, Octadecanoic acid, Butyl 9-tetradecenoate, 7-[3-Chloro-2-hydroxypropyl]guanine, 4-Heneicosanone, 1-cyclopentyl-, 4-Methoxycarbonylmethylundec-3-enedioic acid, dimethyl ester, 5-(4,5-Dihydro-3H-pyrrol-2-ylmethylene)-4,4-dimethylpyrrolidin, Phthalic acid,octyl oct-3-yl ester. The results of anti-fungal activity produced by Callosobruchus maculutus showed that the volatile compounds were highly effective to suppress the growth of Aspergillus niger. Callosobruchus maculutus produce many important secondary metabolites with high biological activities.