Aims of our study were analysis of the secondary metabolite products of Ammi majus and evaluation insecticidal activity against Tribolium castaneum. The identification of bioactive chemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC-MS analysis of Ammi majus revealed the existence of the Pregn-5-ene-3,11-dione, 17,20:20,21-bis [methylenebis(oxy)]-, Penta-2,4-dien-1-one, 5-dimethylamino-1-[5-(4-dimethylamino)], 8-Octadecenal, 9,10-Secocholesta-5,7,10(19)-triene-3,24,25-triol, (3?,5Z,7E)-, 3,7-Diacetamido-7H-s-triazolo[5,1-c]-s-triazole, Piperidine, 2,3-dimethyl-, d-Mannose, 1-Nitro-2-acetamido-1,2-dideoxy-d-mannitol, Paromomycin, d-Glycero-d-ido-heptose, Aminoacetamide, N-methyl-N-[4-(1-pyrrolidinyl)-2-butynyl]-, Pyrrolizin-1,7-dione-6-carboxylic acid, methyl(ester), 5-Allyl-6-methyl-2-phenyl-5,6-dihydro-4H-oxazolo[4,5-c]pyridin-, 3-O-Methyl-d-glucose, Dasycarpidan-1-methanol, acetate (ester) and Corynan-17-ol,18,19-didehydro-10-methoxy-,acetate (ester). Methanolic extract of Ammi majus was active on accumulative mortality of Tribolium castaneum (adult).