Medicinal plants are important source of antibacterial compounds. These plants contain secondary metabolites such as alkaloids, flavonoids, steroids, phenolics, terpenes and volatile oils The objective of this research was to determine the chemical composition of methanolic peel extract. The phytochemical compound screened by GC-MS method. Twentyseven bioactive phytochemical compounds were identified in the methanolic extract of Punica granatum. The identification of phytochemical compounds is based on the peak area, retention time molecular weight, molecular formula, MS Fragment- ions and Pharmacological actions. GC-MS analysis of Punica granatum revealed the existence of the 2H-Pyran,2,2´-[1,10-decanediylbis(oxy)]bis[tetrahydro-, 6-Oxa-bicyclo[3.1.0]hexan-3-one, 2,5-Furandione, 3-methyl-, 2-Furancarboxaldehyde,5-methyl, D-Glucose, 6-O-?-D-galactopyranosyl, D-Limonene, Lactose, DL-Arabinose, 5-Methyl -2- pyrazinylmethanol, 6-Acetyl-?-d-mannose, ?-D-Glucopyranoside, O-?-D-glucopyranosyl-(1.fwdarw)-?-D-fruc, 4-Hexenal,6-hydroxy-4-methyl-,dimethyl acetal, acetate, (Z), 4H-Pyran-4-one, 2,3-dihydro- 3,5-dihydroxy-6-methyl, 4-Chloro-3-n-hexyltetrahydropyran, 4-Methyl itaconate, 5-Hydroxymethylfurfural, 4,6-di-tert-butyl-m-cresol, 3-butyl-4-nitro-pent-4-enoic acid, methyl ester, 1,2-Cyclopentanedicarboxylic acid,4-(1,1-dimethylethyl)-,dimethyl, n-Hexadecanoic acid, Estra-1,3,5(10)-trien-17?-ol, Cis-Vaccenic acid, 9-Octadecenamide, 8,14-Seco-,3,19-epoxyandrostane-8,14-dione,17-acetoxy -3?-meth, Dasycarpidan-1-methanol,acetate(ester), ?-Tocopheryl acetate and ?-Sitosterol. The FTIR analysis of Punica granatum peel proved the presence of Alkenes, Aliphatic fluoro compounds, Alcohols, Ethers, Carboxlic acids, Esters, Nitro Compounds, Alkanes, H-bonded H-X group, Hydrogen bonded Alcohols and Phenols. Punica granatum was highly active against Aspergillus fumigatus (7.00±0.150). Bioactive compounds of Punica granatum was assayed for in vitro antibacterial activity against Proteus mirabilis, Pseudomonas aerogenosa, Escherichia coli, Staphylococcus aureus and Klebsiella pneumonia using the diffusion method in agar. The zone of inhibition were compared with different standard antibiotics. The diameters of inhibition zones ranged from 5.91±0.200 to 1.00±0.110 mm for all treatments. © 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.