Vaccination still the most important strategy in the prevention of infectious diseases, and the developing of powerful vaccine adjuvants is crucial to maximize the efficacy of these vaccines. Our objective was to introduce the laser adjuvant to increase the immunogenicity of Hepatitis B vaccine. Twelve wistar albino male rats were included in this study. The animals were divides into three groups; control, red laser, and infra- red laser groups. Rats were anesthetized by chloroform; the lower dorsal hair of the rats was shaved. The skin of laser groups was exposed next day to red laser (? = 635 nm) or to IR laser (? = 808 nm) for 60 seconds at 300mW after the rats were anesthetized similarly. The rats were then immunized by intradermal (i.d.) administration of (2 ug) of Hepatitis B virus surface antigen (HBsAg) into the laser-illuminated sites. Rats in control group were treated and immunized similarly except for no laser illumination.Laser therapies have diverse immunological effects; the most interesting effect is the change in the differential blood cells count after laser illumination which characterized by a significant increases in lymphocyte count and decreases in neutrophil count after one session of irradiation with red laser light.We found that 635 nm laser induce a significant increases in TNF-? level comparing with the other laser (808 nm) group or the control group. In conclusion the visible red laser has a significant boosting effect on HBsAg vaccine.