This work investigates the rheological behavior of gold nanoparticles filled water (GNPS- water) and filled polyvinyl alcohol (GNPs- PVA) prepared by Nd - YAG laser ablation. Noble metal GNPs were synthesized by pulsed (Q-switched, 1064-Nd: YAG, 8 ns pulse duration and energy E=550mJ and 650mJ laser ablation of gold metal plates immersed in double distilled and deionized water DDDW and PVA solutions. Absorbance spectra of the produced nanoparticles solution was measured by UV-VIS spectrophotometer which show sharp and single peaks 530 nm indicating the produced Au nanoparticles with a narrow size ranging from 10nm to 20 nm with almost spherical shape.The morphology and size was estimated by TEM for GNPs -water .The rheological behavior of GNPs filled water with particle sizes (10 nm) and (20 nm) called nano1 and nano2 and (GNPs- PVA) called nano3 and nano4 respectively were tested using cone –on - plate viscometer.The viscosity, torque, shear stress where measured at different shear rate. The results show that the shear stress increases with the shear rate increasing for nano1, nano2, nano3 and nano4. The viscosity decreased with the shear rate increasing for nano1 , nano2 ,nano3 and nano4.The viscosity for 10 nm concentration is lower than that for 20 nm concentration due to the difference in particles size and energy value