عنوان البحث(Papers / Research Title)
In vitro antibacterial and antifungal activity of Iraqi propolis
الناشر \ المحرر \ الكاتب (Author / Editor / Publisher)
علاء هاني حسن الجراخ
Citation Information
علاء,هاني,حسن,الجراخ ,In vitro antibacterial and antifungal activity of Iraqi propolis , Time 5/17/2011 9:23:42 PM : كلية الطب
وصف الابستركت (Abstract)
فعالية البروبولس العراقي ضد البكتريا والفطريات الممرضة في الزجاج
الوصف الكامل (Full Abstract)
In vitro antibacterial and antifungal activity of Iraqi propolis
Nada K.K. Hendi, Habeeb S. Naher, Alaa H. Al-Charrakh
Abstract:
Propolis is a resinous substance collected by worker bees (Apis mellifera) from the bark of trees and leaves of plants. This salivary and enzymatic secretions-enriched material is used by bees to cover hive walls to ensure a hospital-clean environment. As a natural honeybee hive product, propolis extracts have been used both internally and externally for thousands of years as a healing agent in traditional medicine. Propolis shows a complex chemical composition. Its biological properties- such as antibacterial, antiviral, antifungal, among other activities, have attracted the researchers interest.
The study was aimed at determining the antimicrobial activities of crude ethanolic extract of Al-Museiab propolis (EEMP) against some bacterial and fungal isolates by the method of disc diffusion and agar-well diffusion, respectively. MICs of propolis extracts using the two-fold agar dilution susceptibility method were also determined. Results revealed that Staphylococcus aureuswas higher sensitive to EEMP than other Gram positive and Gram negative bacteria, while standard E. coli strain was highly sensitive to EEMP than other Gram negative bacteria. The effect of EEMP was elevated when the concentration increased to 20% and 30%. EEMP was not effective against C. albicans. Results of disc diffusion methods of crude EEMP at 10% concentration showed that S. aureus was highly sensitive to EEMP inhibition while C. albicans was resistant.
Statistical analysis showed significant differences (P ? 0.05) between results of disc and agar diffusion methods of EEP at concentration of 10%, while there was no significant differences (P ? 0.05) at concentrations of 20% and 30% of extract, respectively. This study concluded that EEP was the most active of all propolis extracts, S. aureus was more sensitive to EEP and AEP than other bacteria, and agar diffusion method was better than disc diffusion method for detection of antimicrobial activity of propolis.
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