عنوان البحث(Papers / Research Title)
Antimicrobial spectrum of the action of bacteriocins from Klebsiella from Hilla/Iraq
الناشر \ المحرر \ الكاتب (Author / Editor / Publisher)
علاء هاني حسن الجراخ
Citation Information
علاء,هاني,حسن,الجراخ ,Antimicrobial spectrum of the action of bacteriocins from Klebsiella from Hilla/Iraq , Time 5/17/2011 9:42:31 PM : كلية الطب
وصف الابستركت (Abstract)
طيف الفعالية ضد المايكروبية للبكتريوسين المنتج من الكليبسيلا في مدينة الحلة
الوصف الكامل (Full Abstract)
Antimicrobial spectrum of the action of bacteriocins from Klebsiella isolates from Hilla/IraqAlaa H. Al-Charrakh, Samira Y. Yousif and Hussein S. Al-Janabi
ABSTRACT:
Background and objectives: Bacteriocins are antibacterial proteins produced by bacteria. They also differ from traditional antibiotics in having a relatively narrow spectrum of action and being lethal only for bacteria which are closely related to the producing strains. The aim of this study was to investigate the antibacterial spectrum of the action of bacteriocins from environmental and clinical Klebsiella pneumoniae isolates from Hilla/Iraq.
Materials and Methods: The antibacterial activity of bacteriocins (klebocins) from Klebsiella pneumoniae isolates against different pathogenic species of Gram-negative and Gram-positive bacteria (by cup assay method) was determined.
Results and Conclusion: The results revealed that brain heart infusion agar medium supplemented with 5 % glycerol was the best culture medium used for detection of klebocin-producing isolates and cup assay was the best method used for such purpose. Results also revealed that klebocins of Klebsiella isolates had a broad antimicrobial spectrum and active, in addition to Klebsiella strains, on many pathogenic species of Gram-negative and some Gram-positive bacteria. Agarose gel electrophoresis of DNA samples of some selected klebocin-producing Klebsiella isolates showed that they harbor plasmid bands different in size and position. Results of conjugation experiments revealed that genes encoding for the production of klebocin were located on conjugative plasmids. The heterologus activity of klebocins on many pathogenic species of Gram-negative and some Gram-positive bacteria especially those isolated from patients with chronic pyelonephritis and chronic otitis media, may indicate that these klebocins could be used as alternative to the broad-spectrum antibiotics.
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